User's guide on how to use the eHSCpr Web server to obtain predictive tags (early stage of hematopoietic stem cell development).
(1) Please provide a data set in CSV format and at least eight gene expressions, as shown in the table.
CSV Format:
| Label |
gene1 |
gene2 |
gene3 |
... |
| 1 |
2 |
3 |
4 |
6 |
| 2 |
3 |
3 |
5 |
6 |
| 3 |
5 |
6 |
4 |
7 |
Example:
Label,Tpt1,S100a16,Gnas,mt.Nd2,Aplnr,mt.Co3,mt.Co2,Gm10076,Kdr,Nfib
1,0.022,0.973,-0.036,-0.342,0.472,-0.314,0.715,-0.632,-0.378,-0.001
1,0.128,-0.575,0.308,0.873,-0.456,-0.206,1.155,0.083,-0.519,-0.474
4,-0.2514,-0.4459,-0.3504,-0.4135,-0.4555,-0.0078,-0.3999,-0.7626,-0.5195,-0.5437
4,-0.312,-0.575,-0.207,-0.27,-0.456,-0.494,0.015,-0.437,-0.519,-0.474
3,-0.7823,-0.4459,-0.6079,-0.4135,0.704,0.46,1.5702,-1.0229,-0.5195,-0.0012
3,-0.7823,-0.4459,-0.6079,-0.4135,0.704,0.46,1.5702,-1.0229,-0.5195,-0.0012
2,-0.8278,-0.1878,-0.6079,-0.9138,-0.4555,-0.6016,-0.6332,-0.7626,-0.3784,-0.584
2,-0.7975,-0.3169,-0.4934,-0.9853,-0.4555,-1.1773,-1.1776,-0.6324,-0.3784,-0.7819
0,0.3402,0.0702,-0.3504,0.5158,0.2402,0.8018,0.5851,-0.0467,0.7503,0.6102
5,-0.2665,-0.5749,-0.4934,-0.056,-0.4555,-0.8895,-0.9443,0.539,-0.5195,-0.3572
5,-0.3424,-0.5749,-0.5507,-0.2705,-0.4555,-0.2777,-0.7369,0.6041,-0.5195,-0.143
0,0.3402,0.3283,1.9954,0.6588,0.2402,0.496,0.4296,0.0835,0.7503,0.3923
(2) The csv file must provide a label. If you don't know the sample label, you can write a random number.
(3) Click uoload, you will get the predicted results of the sample.